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Influence of retinoic acid on TBX1 expression in myocardial cells induced by Shh and Fgf8
Miao LIU, Xiaoyan WU, Jiawei XU, Runming JIN
《医学前沿(英文)》 2009年 第3卷 第1期 页码 61-66 doi: 10.1007/s11684-009-0007-8
immune mapped protein 1 (NcIMP1) is a novel vaccine candidate against neosporosis
Xia CUI,Daoyu YANG,Tao LEI,Hui WANG,Pan HAO,Qun LIU
《农业科学与工程前沿(英文)》 2015年 第2卷 第1期 页码 66-72 doi: 10.15302/J-FASE-2015047
关键词: Neospora caninum immune mapped protein 1 (IMP1) vaccine candidate BALB/c mice
PROTECTIVE ROLES OF D1 PROTEIN TURNOVER AND THE XANTHOPHYLL CYCLE IN TOMATO (
Tao LU, Jiazhi LU, Mingfang QI, Zhouping SUN, Yufeng LIU, Tianlai LI
《农业科学与工程前沿(英文)》 页码 262-279 doi: 10.15302/J-FASE-2021383
关键词: D1 turnover photoinhibition photoprotection photosynthesis tomato xanthophyll cycle
《农业科学与工程前沿(英文)》 2021年 第8卷 第2期
D1 protein turnover and the xanthophyll cycle (XC) are important photoprotective mechanisms in plants that operate under adverse conditions. Here, streptomycin sulfate (SM) and dithiothreitol (DTT) were used in tomato plants as inhibitors of D1 protein turnover and XC to elucidate their photoprotective impacts under sub-high temperature and high light conditions (HH, 35°C, 1000 µmol·m-2·s-1). SM and DTT treatments significantly reduced the net photosynthetic rate, apparent quantum efficiency, maximum photochemical efficiency, and potential activity of photosystem II, leading to photoinhibition and a decline in plant biomass under HH. The increase in reactive oxygen species levels resulted in thylakoid membrane lipid peroxidation. In addition, there were increased non-photochemical quenching and decreased chlorophyll pigments in SM and DTT application, causing an inhibition of D1 protein production at both transcriptional and translational levels. Overall, inhibition of D1 turnover caused greater photoinhibition than XC inhibition. Additionally, the recovery levels of most photosynthesis indicators in DTT-treated plants were higher than in SM-treated plants. These findings support the view that D1 turnover has a more important role than XC in photoprotection in tomato under HH conditions.
关键词: D1 turnover / photoinhibition / photoprotection / photosynthesis / tomato / xanthophyll cycle
Hui Xue, Dike Tao, Yuteng Weng, Qiqi Fan, Shuang Zhou, Ruilin Zhang, Han Zhang, Rui Yue, Xiaogang Wang, Zuolin Wang, Yao Sun
《医学前沿(英文)》 2019年 第13卷 第5期 页码 575-589 doi: 10.1007/s11684-019-0693-9
关键词: fracture extracellular matrix dentin matrix protein 1 proteoglycan cartilage
Quan LI MD , Weiming LI MD , Ping ZOU MD , Jian ZHANG BM ,
《医学前沿(英文)》 2009年 第3卷 第3期 页码 309-315 doi: 10.1007/s11684-009-0043-4
关键词: graft vs host disease proteinase-activated receptor murine model hematopoietic stem cell transplantation
proteins reveals a novel human gene, LY6A, which encodes the candidate ortholog of mouse Ly-6A/Sca-1
《医学前沿(英文)》 2023年 第17卷 第3期 页码 458-475 doi: 10.1007/s11684-022-0968-4
关键词: LU domain-containing protein family novel human gene LY6A pituitary tumor biomarker nonsynonymous SNP GPI-anchored protein
转录因子HNF1A、HNF4A和FOXA2调节肝细胞蛋白质N-糖基化 Article
Vedrana Vičić Bočkor,Nika Foglar,Goran Josipović,Marija Klasić,Ana Vujić,Branimir Plavša,Toma Keser,Samira Smajlović,Aleksandar Vojta,Vlatka Zoldoš
《工程(英文)》 2024年 第32卷 第1期 页码 58-69 doi: 10.1016/j.eng.2023.09.019
Hepatocyte nuclear factor 1 alpha (HNF1A), hepatocyte nuclear factor 4 alpha (HNF4A), and forkhead box protein A2 (FOXA2) are key transcription factors that regulate a complex gene network in the liver, creating a regulatory transcriptional loop. The Encode and ChIP-Atlas databases identify the recognition sites of these transcription factors in many glycosyltransferase genes. Our in silico analysis of HNF1A, HNF4A, and FOXA2 binding to the 10 candidate glyco-genes studied in this work confirms a significant enrichment of these transcription factors specifically in the liver. Our previous studies identified HNF1A as a master regulator of fucosylation, glycan branching, and galactosylation of plasma glycoproteins. Here, we aimed to functionally validate the role of the three transcription factors on downstream glyco-gene transcriptional expression and the possible effect on glycan phenotype. We used the state-of-the-art clustered regularly interspaced short palindromic repeats/dead Cas9 (CRISPR/dCas9) molecular tool for the downregulation of the HNF1A, HNF4A, and FOXA2 genes in HepG2 cells—a human liver cancer cell line. The results show that the downregulation of all three genes individually and in pairs affects the transcriptional activity of many glyco-genes, although downregulation of glyco-genes was not always followed by an unambiguous change in the corresponding glycan structures. The effect is better seen as an overall change in the total HepG2 N-glycome, primarily due to the extension of biantennary glycans. We propose an alternative way to evaluate the N-glycome composition via estimating the overall complexity of the glycome by quantifying the number of monomers in each glycan structure. We also propose a model showing feedback loops with the mutual activation of HNF1A–FOXA2 and HNF4A–FOXA2 affecting glyco-genes and protein glycosylation in HepG2 cells.
关键词: Clustered regularly interspaced short palindromic repeats/dead Cas9 (CRISPR/dCas9) Epigenetics Hepatocyte nuclear factor 1 alpha (HNF1A) Hepatocyte nuclear factor 4 alpha (HNF4A) Forkhead box protein A2 (FOXA2) N-glycosylation HepG2 cells
null
《医学前沿(英文)》 2016年 第10卷 第1期 页码 52-60 doi: 10.1007/s11684-016-0433-3
Hepatocellular carcinoma (HCC) is a lethal liver malignancy worldwide. In this study, we reported that protein phosphatase magnesium-dependent 1δ (PPM1D) was highly expressed in the majority of HCC cases (approximately 59%) and significantly associated with high serum α-fetoprotein (AFP) level (P= 0.044). Kaplan-Meier and Cox regression data indicated that PPM1D overexpression was an independent predictor of HCC-specific overall survival (HR, 2.799; 95% CI, 1.346–5.818, P = 0.006). Overexpressing PPM1D promoted cell viability and invasion, whereas RNA interference-mediated knockdown of PPM1D inhibited proliferation, invasion, and migration of cultured HCC cells. In addition, PPM1D suppression by small interfering RNA decreased the tumorigenicity of HCC cells in vivo. Overall, results suggest that PPM1D is a potential prognostic marker and therapeutic target for HCC.
关键词: PPM1D hepatocellular carcinoma prognosis target therapy
Tian Jiang, Yuhui Hou, Tengjiang Zhang, Xudong Feng, Chun Li
《化学科学与工程前沿(英文)》 2019年 第13卷 第3期 页码 554-562 doi: 10.1007/s11705-019-1834-z
关键词: β-glucuronidase enzyme-inorganic hybrid nanoflower biotransformation glycyrrhizin glycyrrtinic acid 3-O-mono-β-D-glucuronide
Qiong CHEN, Qing YU, Yuhu SONG, Peiyuan Li, Ying CHANG, Zhijun WANG, Lifeng LIU, Wei WU, Jusheng LIN
《医学前沿(英文)》 2009年 第3卷 第2期 页码 148-152 doi: 10.1007/s11684-009-0032-7
关键词: gene X-linked inhibitor of apoptosis protein associated factor-1 (XAF1) promoter transcription regulation
Systems understanding of plant–pathogen interactions through genome-wide protein–protein interaction
Hong LI,Ziding ZHANG
《农业科学与工程前沿(英文)》 2016年 第3卷 第2期 页码 102-112 doi: 10.15302/J-FASE-2016100
关键词: plant–pathogen interactions systems biology omics plant immunity protein–protein interaction network
mTOR-targeted cancer therapy: great target but disappointing clinical outcomes, why?
Shi-Yong Sun
《医学前沿(英文)》 2021年 第15卷 第2期 页码 221-231 doi: 10.1007/s11684-020-0812-7
关键词: mTOR cancer therapy resistance GSK3 protein degradation E3 ubiquitin ligase PD-L1
Mengjie LI, Chunbao LI, Shangxin SONG, Xinglian XU, Guanghong ZHOU
《农业科学与工程前沿(英文)》 2018年 第5卷 第3期 页码 362-372 doi: 10.15302/J-FASE-2018206
This study compared proteome profiles and morphological changes of rat jejunum in response to different dietary proteins. Fifty male Sprague-Dawley rats were fed with casein (control), and isolated beef, pork, fish and chicken proteins for 14 days. Proteome analysis, histological observation and PEPT1 quantification of the jejunum were performed. The results indicated that rats fed with chicken proteins had higher PEPT1 mRNA and protein levels (P<0.05) but lower villus height and ratio of villus height to crypt depth (V/C ratio, P<0.05) than those fed with casein and pork protein. Label-free LC-MS/MS indicated that, as compared to casein, intake of chicken protein can regulate oligopeptide transport mainly by upregulating PEPT1 protein expression and reducing dipeptidyl-peptidase activity related to biological oxidation, and can reduce oligopeptide absorption capacity by regulating Hippo signaling pathway. Although intake of beef and fish proteins had no significant effect on PEPT1 expression, they altered several signaling pathways.
关键词: Hippo signaling pathway meat protein PEPT1 proteome analysis rat jejunum
The role of protein kinase C epsilon in neural signal transduction and neurogenic diseases
null
《医学前沿(英文)》 2011年 第5卷 第1期 页码 70-76 doi: 10.1007/s11684-011-0119-9
Protein kinase C epsilon (PKC ?) is one of major isoforms in novel PKC family. Although it has been extensively characterized in the past decade, the role of PKC ? in neuron is still not well understood. Advances in molecular biology have now removed significant barriers to the direct investigation of PKC ? functions in vivo, and PKC ? has been increasingly implicated in the neural biological functions and associated neurogenic diseases. Recent studies have provided important insights into the influence of PKC ? on cortical processing at both the single cell level and network level. These studies provide compelling evidence that PKC ? could regulate distinct aspects of neural signal transduction and suggest that the coordinated actions of a number of molecular signals contribute to the specification and differentiation of PKC ? signal pathway in the developing brain.
关键词: protein kinase C ? signal transduction neurogenic disease
标题 作者 时间 类型 操作
Influence of retinoic acid on TBX1 expression in myocardial cells induced by Shh and Fgf8
Miao LIU, Xiaoyan WU, Jiawei XU, Runming JIN
期刊论文
immune mapped protein 1 (NcIMP1) is a novel vaccine candidate against neosporosis
Xia CUI,Daoyu YANG,Tao LEI,Hui WANG,Pan HAO,Qun LIU
期刊论文
PROTECTIVE ROLES OF D1 PROTEIN TURNOVER AND THE XANTHOPHYLL CYCLE IN TOMATO (
Tao LU, Jiazhi LU, Mingfang QI, Zhouping SUN, Yufeng LIU, Tianlai LI
期刊论文
PROTECTIVE ROLES OF D1 PROTEIN TURNOVER AND THE XANTHOPHYLL CYCLE IN TOMATO (SOLANUM LYCOPERSICUM) UNDER
期刊论文
Glycosylation of dentin matrix protein 1 is critical for fracture healing via promoting chondrogenesis
Hui Xue, Dike Tao, Yuteng Weng, Qiqi Fan, Shuang Zhou, Ruilin Zhang, Han Zhang, Rui Yue, Xiaogang Wang, Zuolin Wang, Yao Sun
期刊论文
Gene and protein expression of proteinase-activated receptor-1, 2 in a murine model of acute graft host
Quan LI MD , Weiming LI MD , Ping ZOU MD , Jian ZHANG BM ,
期刊论文
proteins reveals a novel human gene, LY6A, which encodes the candidate ortholog of mouse Ly-6A/Sca-1
期刊论文
转录因子HNF1A、HNF4A和FOXA2调节肝细胞蛋白质N-糖基化
Vedrana Vičić Bočkor,Nika Foglar,Goran Josipović,Marija Klasić,Ana Vujić,Branimir Plavša,Toma Keser,Samira Smajlović,Aleksandar Vojta,Vlatka Zoldoš
期刊论文
Protein phosphatase magnesium-dependent 1δ is a novel tumor marker and target in hepatocellular carcinoma
null
期刊论文
Construction of a CaHPO4-PGUS1 hybrid nanoflower through protein-inorganic self-assembly, and its application
Tian Jiang, Yuhui Hou, Tengjiang Zhang, Xudong Feng, Chun Li
期刊论文
Cloning of human XAF1 gene promoter and assay of its transcription activity in a variety of cell lines
Qiong CHEN, Qing YU, Yuhu SONG, Peiyuan Li, Ying CHANG, Zhijun WANG, Lifeng LIU, Wei WU, Jusheng LIN
期刊论文
Systems understanding of plant–pathogen interactions through genome-wide protein–protein interaction
Hong LI,Ziding ZHANG
期刊论文
mTOR-targeted cancer therapy: great target but disappointing clinical outcomes, why?
Shi-Yong Sun
期刊论文
Proteome comparisons reveal influence of different dietary proteins on the development of rat jejunum
Mengjie LI, Chunbao LI, Shangxin SONG, Xinglian XU, Guanghong ZHOU
期刊论文